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M. R. Chakraborty , S. Dutta , P. K. Bhattacharya , N. C. Chatterjee

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Aruna Kundu , N. C. Chatterjee

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Three biocontrol agents: Trichoderma viride , Trichoderma lignorum , Trichoderma harzianum were evaluated in-vitro to assess their mode of antagonism against a wood rot fungus Polyporus sanguineus. T. harzianum was the most effective in causing significant suppression of growth of P. sanguine us through competition and through the production of volatile antibiotics followed by T. lignorum and T. viride. Their non-volatile antibiotics suppressed 100% growth of the test fungus. The antagonists were also mycoparasitic on P. sanguineus. Maximum growth inhibition of the test fungus occurred after 7 days of incubation. The biocontrol agents appeared to have potential for application in practical and economic control of bamboo rot by Polyporus sanguine us.

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R. N. Medda , Sikha Dutta , N. C. Chatterjee

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N. C. Chatterjee

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N. C. Chatterjee

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N. C. Chatterjee , P. N. Chatterjee

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For some years now the Branch of Forest Entomology has been engaged in evolving suitable prophylactic methods of treatment of freshly felled timber in Indian forests, as the loss caused in the first few days or weeks of felling and before the logs are transported to the sites of conversion or utilization are very considerable. There is much demand for information regarding prophylactic methods both from forest departments and other government departments including the defence departments, as well as from private concerns. Our approach has been two-fold: first, to devise "natural or silvicultural" methods of storage in forests; secondly, by the use of insecticides. "Natural" methods, by their very nature must vary from one timber species to another, as also with the region, and we have consequently taken up three investigation with 3 or 4 common species in the Madras region, the Bombay region and the Dehra Dun (wet, North India) region. The results of the Madras investigation have already been published (Khan, 1947), those of the Bombay investigations are presented here, while those of the Dehra Dun investigations are under study. One of the authors, the late Dr. N. C. Chatterjee, supervised the investigation in its later stages. The manuscript of the paper has been written by Dr. P. N. Chatterjee and revised by the undersigned; Dr. N. C. Chatterjee unfortunately did not live to see it. Reference is also invited to a note by the undersigned, to be shortly published ( Roonwal, 1951 ), in which are given practical directions on prophylactic treatments both by the natural" methods and by the use of insecticides.

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D. Bhattacharya ¹, M. R. Chakraborty ², N. C. Chatterjee ³, C. Sengupta ¹

Affiliations
1 Department of Botany, Kalyani University, Kalyani (W.B.), IN
2 Department of Botany, Bankura Christian College, Bankura (W.B.), IN
3 Department of Botany, Burdwan University, Burdwan (W.B.), IN

Abstract

Alternaria sp attacks Vigna radiata causes many losses in yield. The antagonistic efficiency of Trichoderma spp evaluated in in vitro and in in vivo study against blight disease of Vigna. Trichoderma had shown significant antagonistic activity against Alternaria. Trichoderma species were capable of producing some volatile and nonvolatiles substances which inhibit the growth of the pathogen. Effect of non-volatile substances seems to be more effective on mycelial growth of the pathogen. T. viride was most promising to check the radial growth of the pathogen (100%) wherein T. harzianum exhibited 97.78 per cent inhibition. Studies on the production of hydrolytic enzymes viz., β-1, 3 glucanase and chitinase by four Trichoderma spp were also studied. Field trials with the application of two Trichoderma species were made where T. viride caused reduction of infection of plants to 54.11per cent.

Keywords

Alternaria, Chitinase, Laminarinase, Non-Volatile, Volatile, Trichoderma spp. Vigna.

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